Product Description |
Recombinant Human TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Pro141. |
Product Background |
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells. |
Product Category |
Recombinant Protein |
Target |
TIGIT |
Synonyms |
TIGIT; VSIG9; VSTM3; WUCAM |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met22-Pro141 |
Molecular Weight |
The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. The affinity constant of 0.17uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17uM as determined in a SPR assay (Biacore T200). |
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Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. |
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Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). |
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Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46ug/ml. |
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Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC. |
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