Human TIGIT Protein (TIG-HM110)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.17uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met22-Pro141

Catalog: TIG-HM110

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Product Description	Recombinant Human TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Pro141.
Product Background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Product Category	Recombinant Protein

Target	TIGIT
Synonyms	TIGIT; VSIG9; VSTM3; WUCAM
Gene/Protein ID	Q495A1-1
Species	Human
Exact Sequence	Met22-Pro141
Molecular Weight	The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.17uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17uM as determined in a SPR assay (Biacore T200).
	Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test).
	Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46ug/ml.
	The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
	Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.