Name: Human TIGIT Protein (TIG-HM110)
Brand: KactusBio
SKU: TIG-HM110-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA. The affinity constant of 0.17uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met22-Pro141

Catalog #TIG-HM110

Size

100UG

500UG

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Human TIGIT Protein (TIG-HM110) Summary

Product Description	Recombinant Human TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Pro141.
Product Background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human TIGIT Protein (TIG-HM110) Specifications/Details

Protein	TIGIT
Synonyms	TIGIT; VSIG9; VSTM3; WUCAM
Accession	Q495A1-1
Species	Human
Biotinylated	no
Amino Acid Range	Met22-Pro141
Molecular Weight	The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA. The affinity constant of 0.17uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17uM as determined in a SPR assay (Biacore T200).
	Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA.
	Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
	Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46ug/ml.
	The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.

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