Human TIGIT Protein (TIG-HM110)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 18ng/ml determined by ELISA. The affinity constant of 0.17μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met22-Pro141

Catalog: TIG-HM110

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Product Description Recombinant Human TIGIT Protein is expressed from Expi293 with His tag at the C-terminal.It contains Met22-Pro141.
Product Category Recombinant Protein
Target TIGIT
Synonyms TIGIT; VSIG9; VSTM3; WUCAM
Gene/Protein ID Q495A1-1
Species Human
Exact Sequence Met22-Pro141
Molecular Weight The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 18ng/ml determined by ELISA. The affinity constant of 0.17μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17μM as determined in a SPR assay (Biacore T200).
Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 18ng/ml determined by ELISA.
Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46μg/ml.
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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