Product Description |
Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile56-Glu182. |
Product Background |
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. |
Product Category |
Recombinant Protein |
Target |
TRAIL R2/DR5/TNFRSF10B |
Synonyms |
CD262; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRICK2; TRICK2A; TRICKB; ZTNFR9; KILLER |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile56-Glu182 |
Molecular Weight |
The protein has a predicted MW of 41.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Trail, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Tris, 100mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Trail, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test). |
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Human TRAIL R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TRAIL R2 is greater than 95% as determined by SEC-HPLC. |
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