Human TRAIL/TNFRSF10 Protein (TRL-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human TRAIL at 2_g/ml (100_l/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA. The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val114-Gly281

Catalog: TRL-HE001

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Product Description Recombinant Human TRAIL/TNFRSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281.
Product Category Recombinant Protein
Target TRAIL/TNFRSF10
Synonyms Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2
Gene/Protein ID NP_003801.1
Species Human
Exact Sequence Val114-Gly281
Molecular Weight The protein has a predicted MW of 19.6 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human TRAIL at 2_g/ml (100_l/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA. The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DcR1, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
Immobilized Human TRAIL at 2_g/ml (100_l/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA.
Immobilized Human TRAIL, No Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.27ug/ml determined by ELISA.
The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC.
Human TRAIL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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