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Human TRAIL/TNFSF10 Protein (active trimer) (TRL-HE001) Summary
Product Description |
Recombinant Human TRAIL/TNFSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281. |
Product Background |
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human TRAIL/TNFSF10 Protein (active trimer) (TRL-HE001) Specifications/Details
Protein |
TRAIL/TNFSF10 |
Synonyms |
Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val114-Gly281 |
Molecular Weight |
The protein has a predicted MW of 19.6 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 300mM NaCl (pH 7.2). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human TRAIL R3, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). |
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Immobilized Human TRAIL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. |
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Human TRAIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TRAIL is greater than 95% and the molecular weight of this protein is around 45-65 kDa as determined by SEC-MALS. |
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The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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