Human Transferrin R/CD71 Protein (TFR-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.1uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Cys89-Phe760
Catalog #TFR-HM201
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Product Description
 Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys89-Phe760.
Product Background
 The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Product Category
 Recombinant Protein
Target
 Transferrin R/CD71
Synonyms
 TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Cys89-Phe760
Molecular Weight
 The protein has a predicted MW of 101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.1uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Transferrin R, hFc Tag captured on CM5 Chip via Anti-Fc Antibody can bind Human Transferrin, His Tag with an affinity constant of 0.1uM as determined in SPR assay (Biacore T200).
Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test).
Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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