Regular price
$247.50
Order by 4pm ET for delivery on:
Human Transferrin R/CD71 Protein (TFR-HM201) Summary
Product Description |
Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Cys89-Phe760. |
Product Background |
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human Transferrin R/CD71 Protein (TFR-HM201) Specifications/Details
Protein |
Transferrin R/CD71 |
Synonyms |
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys89-Phe760 |
Molecular Weight |
The protein has a predicted MW of 101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Transferrin, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Transferrin R, hFc Tag with the EC50 of 58.4ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Biotinylated Human Transferrin, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Transferrin R, hFc Tag with the EC50 of 58.4ng/ml determined by ELISA. |
|
|
Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA. |
|
|
Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
