Human Transferrin R/CD71 Protein (TFR-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.1uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Cys89-Phe760

Catalog: TFR-HM201

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Product Description	Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys89-Phe760.
Product Background	The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Product Category	Recombinant Protein

Target	Transferrin R/CD71
Synonyms	TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Gene/Protein ID	P02786-1
Species	Human
Exact Sequence	Cys89-Phe760
Molecular Weight	The protein has a predicted MW of 101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.1uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human Transferrin R, hFc Tag captured on CM5 Chip via Anti-Fc Antibody can bind Human Transferrin, His Tag with an affinity constant of 0.1uM as determined in SPR assay (Biacore T200).
	Immobilized Human Transferrin R, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA (QC Test).
	Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.