Human TREM2 Protein (TRM-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human TREM2, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA. The affinity constant of 1.004 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: His19-Ser174

Catalog: TRM-HM201

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Product Description Recombinant Human TREM2 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains His19-Ser174.
Product Category Recombinant Protein
Target TREM2
Synonyms PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Gene/Protein ID Q9NZC2-1
Species Human
Exact Sequence His19-Ser174
Molecular Weight The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TREM2, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA. The affinity constant of 1.004 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 1.004 μM as determined in SPR assay (Biacore T200).
Immobilized Human TREM2, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA.
Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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