Human TREM2 Protein (TRM-HM201)
Human TREM2 Protein (TRM-HM201)
Human TREM2 Protein (TRM-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: His19-Ser174
Catalog #TRM-HM201
Regular price $203.00
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Product Description
Recombinant Human TREM2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His19-Ser174.
Product Background
TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2- consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.
Product Category
Recombinant Protein
Target
TREM2
Synonyms
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Accession
Species
Human
Biotinylated
no
Amino Acid Range
His19-Ser174
Molecular Weight
The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE3, His Tag with an affinity constant of 1.21 uM as determined in SPR assay (Biacore T200).
Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test).
Human TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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