Name: Human TREM2 Protein (TRM-HM201)
Brand: KactusBio
SKU: TRM-HM201-100UG
Price: 223.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: His19-Ser174

Catalog #TRM-HM201

Size

100UG

500UG

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Product Description	Recombinant Human TREM2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His19-Ser174.
Product Background	TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2- consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.
Product Category	Recombinant Protein / Antibody discovery

Protein	TREM2
Synonyms	PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Accession	Q9NZC2-1
Species	Human
Biotinylated	no
Amino Acid Range	His19-Ser174
Molecular Weight	The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE3, His Tag with an affinity constant of 1.21 uM as determined in SPR assay (Biacore T200).
	Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test).
	Human TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.