Product Description |
Recombinant Human TROP-2/TACSTD2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His27-Thr274. |
Product Background |
Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. |
Product Category |
Recombinant Protein |
Target |
TROP-2/TACSTD2 |
Synonyms |
EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His27-Thr274 |
Molecular Weight |
The protein has a predicted MW of 54.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TROP-2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TROP-2 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human TROP-2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TROP-2 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. |
|
|
Human TROP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TROP-2 is greater than 95% as determined by SEC-HPLC. |
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