Mouse AXL Protein (AXL-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GAS6, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: His20-Trp445

Catalog: AXL-MM101

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Product Description Recombinant Mouse AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His20-Trp445.

Product Background

Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Product Category

Recombinant Protein

Target

AXL
Synonyms Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1

Gene/Protein ID

NP_033491.2

Species

Mouse

Exact Sequence

His20-Trp445
Molecular Weight The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GAS6, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GAS6, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test).
The purity of Mouse AXL is greater than 95% as determined by SEC-HPLC.
Mouse AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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