Mouse AXL Protein (AXL-MM101) Summary
Product Description |
Recombinant Mouse AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His20-Trp445. |
Product Background |
Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse AXL Protein (AXL-MM101) Specifications/Details
Protein |
AXL |
Synonyms |
Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
His20-Trp445 |
Molecular Weight |
The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). |
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Mouse AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse AXL is greater than 95% as determined by SEC-HPLC. |
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