Mouse AXL Protein (AXL-MM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GAS6, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: His20-Trp445

Catalog: AXL-MM101

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Product Description	Recombinant Mouse AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His20-Trp445.
Product Category	Recombinant Protein

Target	AXL
Synonyms	Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Gene/Protein ID	NP_033491.2
Species	Mouse
Exact Sequence	His20-Trp445
Molecular Weight	The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GAS6, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GAS6, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test).
	The purity of Mouse AXL is greater than 95% as determined by SEC-HPLC.
	Mouse AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.