Mouse BCMA/TNFRSF17 Protein (BCM-MM217)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test). The affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met1-Thr49

Catalog: BCM-MM217

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Product Description	Recombinant Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met1-Thr49.
Product Background	B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
Product Category	Recombinant Protein

Target	BCMA/TNFRSF17
Synonyms	CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
Gene/Protein ID	O88472-1
Species	Mouse
Exact Sequence	Met1-Thr49
Molecular Weight	The protein has a predicted MW of 31 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test). The affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test).
	The purity of Mouse BCMA is greater than 95% as determined by SEC-HPLC.
	Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.