Mouse BCMA/TNFRSF17 Protein (BCM-MM417)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse BCMA, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met1-Thr49

Catalog: BCM-MM417

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Product Description Recombinant Mouse BCMA/TNFRSF17 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Met1-Thr49.
Product Category Recombinant Protein
Target BCMA/TNFRSF17
Synonyms CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
Gene/Protein ID O88472-1
Species Mouse
Exact Sequence Met1-Thr49
Molecular Weight The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse BCMA, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 μM as determined in SPR assay (Biacore T200).
Immobilized Mouse BCMA, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA.
The purity of Mouse BCMA is greater than 95% as determined by SEC-HPLC.
Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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