Mouse BCMA/TNFRSF17 Protein (BCM-MM417)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Mouse BCMA, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 93.9ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met1-Thr49

Catalog: BCM-MM417

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Product Description Recombinant Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49.

Product Background

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Product Category

Recombinant Protein

Target

BCMA/TNFRSF17
Synonyms CD269; TNFRSF17; BCMA; BCM; TNFRSF13A

Gene/Protein ID

O88472-1

Species

Mouse

Exact Sequence

Met1-Thr49
Molecular Weight The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse BCMA, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 93.9ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse BCMA, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 93.9ng/ml determined by ELISA (QC Test).
Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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