Mouse BCMA/TNFRSF17 Protein (BCM-MM417)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse BCMA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met1-Thr49
Catalog #BCM-MM417
Regular price $379.50
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Product Description

Recombinant Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49.

Product Background

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

BCMA/TNFRSF17

Synonyms

CD269; TNFRSF17; BCMA; BCM; TNFRSF13A

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Met1-Thr49

Molecular Weight

The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse BCMA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse BCMA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). Immobilized Mouse BCMA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test).
Mouse BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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