Mouse BCMA/TNFRSF17 Protein (BCM-MM417)
Catalog: BCM-MM417
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| Product Description | Recombinant Mouse BCMA/TNFRSF17 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Met1-Thr49. |
| Product Category | Recombinant Protein |
| Target | BCMA/TNFRSF17 |
| Synonyms | CD269; TNFRSF17; BCMA; BCM; TNFRSF13A |
| Gene/Protein ID | O88472-1 |
| Species | Mouse |
| Exact Sequence | Met1-Thr49 |
| Molecular Weight | The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse BCMA, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse BCMA, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. |
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The purity of Mouse BCMA is greater than 95% as determined by SEC-HPLC. |
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Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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