Mouse CD47 Protein (CD7-MM147)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: The affinity constant of 1.33 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln19-Lys140

Catalog: CD7-MM147

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Product Description Recombinant Mouse CD47 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Gln19-Lys140.
Product Category Recombinant Protein
Target CD47
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Gene/Protein ID Q61735-1
Species Mouse
Exact Sequence Gln19-Lys140
Molecular Weight The protein has a predicted MW of 14.9 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.33 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 μM as determined in SPR assay (Biacore T200).
The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC.
Mouse CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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