Mouse CD73/NT5E Protein (CD7-MM173)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Trp29-Lys549

Catalog: CD7-MM173

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Product Description	Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549.
Product Background	CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
Product Category	Recombinant Protein

Target	CD73/NT5E
Synonyms	CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Gene/Protein ID	Q61503
Species	Mouse
Exact Sequence	Trp29-Lys549
Molecular Weight	The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test).
	Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is >10000 pmol/min/ug (QC Test).
	The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC.
	Mouse CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.