Mouse CD73/NT5E Protein (CD7-MM173)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp29-Lys549

Catalog: CD7-MM173

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Product Description Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549.

Product Background

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.

Product Category

Recombinant Protein

Target

CD73/NT5E
Synonyms CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Accession

Species

Mouse

Amino Acid Range

Trp29-Lys549
Molecular Weight The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test).
The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC.
Mouse CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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