Mouse CD73/NT5E Protein (CD7-MM173)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is >10000 pmol/min/ug (QC Test).
Amino Acid Range: Trp29-Lys549

Catalog #CD7-MM173

Price

$297⁰⁰

Size

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Mouse CD73/NT5E Protein (CD7-MM173) Summary

Product Description	Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549.
Product Background	CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.

Protein	CD73/NT5E
Synonyms	CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA;5NT; EC3.1.3.5
Accession	Q61503
Species	Mouse
Biotinylated	no
Amino Acid Range	Trp29-Lys549
Molecular Weight	The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is >10000 pmol/min/ug (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test).
	Mouse CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC.