Product Description |
Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549. |
Product Background |
CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. |
Product Category |
Recombinant Protein |
Target |
CD73/NT5E |
Synonyms |
CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Trp29-Lys549 |
Molecular Weight |
The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). |
|
|
Mouse CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC. |
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