Mouse CD73/NT5E Protein (CD7-MM173)
Mouse CD73/NT5E Protein (CD7-MM173)
Mouse CD73/NT5E Protein (CD7-MM173)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp29-Lys549
Catalog #CD7-MM173
Regular price $270.00
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Product Description

Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549.

Product Background

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.

Product Category

Recombinant Protein / Antibody discovery

Protein

CD73/NT5E

Synonyms

CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Trp29-Lys549

Molecular Weight

The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). Immobilized Mouse CD73, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test).
Mouse CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC.

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