Name: Mouse EGFR/HER1 Protein (EGF-MM101)
Brand: KactusBio
SKU: EGF-MM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 3.98 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu25-Ser647

Catalog #EGF-MM101

Size

100UG

500UG

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Mouse EGFR/HER1 Protein (EGF-MM101) Summary

Product Description	Recombinant Mouse EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Ser647.
Product Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets \| ADC targets

Mouse EGFR/HER1 Protein (EGF-MM101) Specifications/Details

Protein	EGFR/HER1
Synonyms	ErbB; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	Q01279
Species	Mouse
Biotinylated	no
Amino Acid Range	Leu25-Ser647
Molecular Weight	The protein has a predicted MW of 70.40 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 3.98 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 3.98 nM as determined in SPR assay (Biacore T200).
	Mouse EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse EGFR is greater than 95% as determined by SEC-HPLC.

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