Mouse FAP Protein (FAP-MM101)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse FAP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu26-Asp761

Catalog: FAP-MM101

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Product Description Recombinant Mouse FAP Protein is expressed from Expi293 with His tag at the N-terminal.It contains Leu26-Asp761.
Product Category Recombinant Protein
Target FAP
Synonyms FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Gene/Protein ID P97321-1
Species Mouse
Exact Sequence Leu26-Asp761
Molecular Weight The protein has a predicted MW of 86.4 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse FAP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse FAP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA.
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1800 pmol/min/µg.
The purity of Mouse FAP is greater than 95% as determined by SEC-HPLC.
Mouse FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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