• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Val28-Pro674
Catalog #GAS-MM106
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Mouse GAS6 Protein (GAS-MM106) Summary

Product Description

 Recombinant Mouse GAS6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val28-Pro674.

Product Background

 Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse GAS6 Protein (GAS-MM106) Specifications/Details

Protein
 GAS6
Synonyms
 GAS-6; AXLLG; GAS6; DKFZp666G247; FLJ34709
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Val28-Pro674
Molecular Weight
 The protein has a predicted MW of 73.09 kDa. Due to glycosylation, the protein migrates to 74-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200).
Mouse TYRO3, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 11.31 uM as determined in SPR assay (Biacore T200). Mouse TYRO3, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 11.31 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test). Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test).
Mouse GAS6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse GAS6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GAS6 is greater than 95% as determined by SEC-HPLC. The purity of Mouse GAS6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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