Mouse GAS6 Protein (GAS-MM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val28-Pro674
Catalog #GAS-MM106
Regular price $315.00
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Product Description
Recombinant Mouse GAS6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val28-Pro674.
Product Background
Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).
Product Category
Recombinant Protein
Target
GAS6
Synonyms
GAS-6; AXLLG; GAS6; DKFZp666G247; FLJ34709
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val28-Pro674
Molecular Weight
The protein has a predicted MW of 73.09 kDa. Due to glycosylation, the protein migrates to 74-90 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test). The affinity constant of 15.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Mouse GAS6, His Tag at 5ug/m l (100ul/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA (QC Test).
Mouse GAS6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GAS6 is greater than 95% as determined by SEC-HPLC.

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