Mouse HGF R/c-MET Protein (MET-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu25-Ala931

Catalog: MET-MM101

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Product Description Recombinant Mouse HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu25-Ala931.

Product Background

c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Product Category

Recombinant Protein

Target

HGF R/c-MET

Gene/Protein ID

P16056

Species

Mouse

Exact Sequence

Glu25-Ala931
Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.1 kDa (α chain) and 69.8 kDa (β chain). Due to glycosylation, the protein migrates to 40-50 kDa (α subunit) and 78-98 kDa (β subunit) based on Tris-Bis PAGE result.

Activity

Immobilized Mouse HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.
The purity of Mouse HGF R is greater than 95% as determined by SEC-HPLC.
Mouse HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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