- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Mouse IGF1R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 2.3ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Glu31-His936
Product Description |
Recombinant Mouse IGF1R/CD221 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-His936. |
Product Background |
The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target. |
Product Category |
Recombinant Protein |
Target |
IGF1R/CD221 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu31-His936 |
Molecular Weight |
The protein has a predicted MW of 80.72 kDa (alpha subunit) and 23.0 kDa (beta subunit). Due to furin cleavage and glycosylation, the protein migrates to 110-140 kDa(alpha subunit) and 50-65 kDa(beta subunit) based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IGF1R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 2.3ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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