Mouse LAG3/CD223 Protein (LAG-MM131)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Mouse LAG3 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse FGL1, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser23-Leu442

Catalog: LAG-MM131

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Product Description Recombinant Mouse LAG3/CD223 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Ser23-Leu442 .
Product Category Recombinant Protein
Target LAG3/CD223
Synonyms CD223; FDC; LAG3; LAG-3; sLAG-3
Gene/Protein ID Q61790
Species Mouse
Exact Sequence Ser23-Leu442
Molecular Weight The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse LAG3 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse FGL1, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse LAG3 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse FGL1, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA.
Mouse LAG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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