SEC-HPLC chromatogram for protein sample with main peak at 7.837 min, demonstrating molecular size homogeneity and purity.
Mouse MSLN/Mesothelin Protein (MSL-MM180)
Mouse MSLN/Mesothelin Protein (MSL-MM180)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse MSLN, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp298-Ser600
Catalog #MSL-MM180
Regular price $346.50
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Product Description

Recombinant Mouse MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp298-Ser600.

Product Background

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF).

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

MSLN/Mesothelin

Synonyms

MSLN; CAK1 antigen; CAK1; Mesothelin; MPF; MPFSMRP; SMR;

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Asp298-Ser600

Molecular Weight

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse MSLN, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse MSLN, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 21.7ng/ml determined by ELISA. Immobilized Mouse MSLN, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 21.7ng/ml determined by ELISA.
Mouse MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse MSLN is greater than 95% as determined by SEC-HPLC. The purity of Mouse MSLN is greater than 95% as determined by SEC-HPLC.

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