Product Description |
Recombinant Mouse Transferrin R/CD71 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Cys89-Phe763. |
Product Background |
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
Product Category |
Recombinant Protein |
Target |
Transferrin R/CD71 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Cys89-Phe763 |
Molecular Weight |
The protein has a predicted MW of 76.99 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse Transferrin R is greater than 95% as determined by SEC-HPLC. |
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