Mouse VEGF R2/KDR Protein (VGF-MM1R2)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse VEGFR2, His Tag with the EC50 of 1.15ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Glu762

Catalog: VGF-MM1R2

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Product Description Recombinant Mouse VEGF R2/KDR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Glu762.

Product Background

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.

Product Category

Recombinant Protein

Target

VEGF R2/KDR
Accession

Species

Mouse

Amino Acid Range

Ala20-Glu762
Molecular Weight The protein has a predicted MW of 84.11 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse VEGFR2, His Tag with the EC50 of 1.15ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse VEGFR2, His Tag with the EC50 of 1.15ug/ml determined by ELISA.
The purity of Mouse VEGF R2 is greater than 95% as determined by SEC-HPLC.
Mouse VEGF R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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