Rat CD40/TNFRSF5 Protein (CD4-RM140)

Immobilized Rat CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand (Trimer), monomeric Fc Tag (Cat. CDL-MM240) with the EC50 of 2.4ng/ml determined by ELISA.

Rat CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

The purity of Rat CD40 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand (Trimer), monomeric Fc Tag (Cat. CDL-MM240) with the EC50 of 2.4ng/ml determined by ELISA.
Amino Acid Range: Val24-Arg193

Catalog #CD4-RM140

Price

$264⁰⁰

Size

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Rat CD40/TNFRSF5 Protein (CD4-RM140) Summary

Product Description

Recombinant Rat CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val24-Arg193.

Product Background

CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.

Rat CD40/TNFRSF5 Protein (CD4-RM140) Specifications/Details

Protein	CD40/TNFRSF5
Synonyms	CD40; CD40L receptor;- TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013;
Accession	A6JXD6
Species	Rat
Biotinylated	no
Amino Acid Range	Val24-Arg193
Molecular Weight	The protein has a predicted MW of 20.39 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Rat CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand (Trimer), monomeric Fc Tag (Cat. CDL-MM240) with the EC50 of 2.4ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Rat CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand (Trimer), monomeric Fc Tag (Cat. CDL-MM240) with the EC50 of 2.4ng/ml determined by ELISA.
	Rat CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Rat CD40 is greater than 95% as determined by SEC-HPLC.