Rat CD47 Protein (CD7-RM247)
Rat CD47 Protein (CD7-RM247)
Rat CD47 Protein (CD7-RM247)
Rat CD47 Protein (CD7-RM247)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse SIRP alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln19-Lys140
Catalog #CD7-RM247
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Product Description
 Recombinant Rat CD47 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln19-Lys140.
Product Background
 CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Product Category
 Recombinant Protein
Target
 CD47
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Gln19-Lys140
Molecular Weight
 The protein has a predicted MW of 40.40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse SIRP alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse SIRP alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA.
Rat CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat CD47 is greater than 95% as determined by SEC-HPLC.
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