Rat CD47 Protein (CD7-RM247)
Rat CD47 Protein (CD7-RM247)
Rat CD47 Protein (CD7-RM247)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse SIRP alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln19-Lys140
Catalog #CD7-RM247
Regular price $264.00
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Product Description

Recombinant Rat CD47 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln19-Lys140.

Product Background

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

CD47

Synonyms

CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6

Accession

Species

Rat

Biotinylated

no

Amino Acid Range

Gln19-Lys140

Molecular Weight

The protein has a predicted MW of 40.40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse SIRP alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse SIRP alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test). Immobilized Mouse SIRP alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test).
Rat CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat CD47 is greater than 95% as determined by SEC-HPLC. The purity of Rat CD47 is greater than 95% as determined by SEC-HPLC.

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