Rat MSLN/Mesothelin Protein (MSL-RM180)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asp298-Ser600

Catalog: MSL-RM180

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Product Description	Recombinant Rat MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp298-Ser600.
Product Background	Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF).
Product Category	Recombinant Protein

Target	MSLN/Mesothelin
Gene/Protein ID	XP_032768340.1
Species	Rat
Exact Sequence	Asp298-Ser600
Molecular Weight	The protein has a predicted MW of 35.67 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Rat MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Rat MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 17.1ng/ml determined by ELISA.
	The purity of Rat MSLN is greater than 95% as determined by SEC-HPLC.
	Rat MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.