Product Description |
Recombinant Rat MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp298-Ser600. |
Product Background |
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). |
Product Category |
Recombinant Protein |
Target |
MSLN/Mesothelin |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Asp298-Ser600 |
Molecular Weight |
The protein has a predicted MW of 35.67 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rat MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Rat MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 17.1ng/ml determined by ELISA. |
|
|
Rat MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Rat MSLN is greater than 95% as determined by SEC-HPLC. |
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