• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala23-Thr696
Catalog #CCK-RM104
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Rat PTK7/CCK4 Protein (CCK-RM104) Summary

Product Description

 Recombinant Rat PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala23-Thr696.

Product Background

 Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation, highlighting the important regulatory role of PTK7 protein in these processes. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling.

Rat PTK7/CCK4 Protein (CCK-RM104) Specifications/Details

Protein
 PTK7/CCK4
Synonyms
 CCK-4; PTK7;CCK4
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Ala23-Thr696
Molecular Weight
 The protein has a predicted MW of 75.95 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Rat PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat PTK7 is greater than 95% as determined by SEC-HPLC. The purity of Rat PTK7 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...