Product Description |
Recombinant Rhesus macaque DLL3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala27-Arg490. |
Product Background |
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). |
Product Category |
Recombinant Protein |
Protein |
DLL3 |
Synonyms |
Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 |
Accession |
|
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Ala27-Arg490 |
Molecular Weight |
The protein has a predicted MW of 51 kDa. Due to glycosylation,the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rhesus macaque DLL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 50mM NaCl, 200mM L-arginine (pH 8.5) / PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Rhesus macaque DLL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. |
|
|
Rhesus macaque DLL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Rhesus macaque DLL3 is greater than 95% as determined by SEC-HPLC. |
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