- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Leu25-Ser645
Product Description |
Recombinant Rhesus macaque EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Ser645. |
Product Background |
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |
Product Category |
Recombinant Protein |
Target |
EGFR/HER1 |
Synonyms |
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
Accession |
|
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Leu25-Ser645 |
Molecular Weight |
The protein has a predicted MW of 69.8 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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