Rhesus macaque EGFR/HER1 Protein (EGF-CM101)
Rhesus macaque EGFR/HER1 Protein (EGF-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu25-Ser645
Catalog #EGF-CM101
Regular price $247.50
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Product Description

Recombinant Rhesus macaque EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Ser645.

Product Background

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

EGFR/HER1

Synonyms

ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

Accession

Species

Rhesus macaque

Biotinylated

no

Amino Acid Range

Leu25-Ser645

Molecular Weight

The protein has a predicted MW of 69.8 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test).
Rhesus macaque EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rhesus macaque EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque EGFR is greater than 95% as determined by SEC-HPLC. The purity of Rhesus macaque EGFR is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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