Rhesus macaque EGFR/HER1 Protein (EGF-CM101)
Rhesus macaque EGFR/HER1 Protein (EGF-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu25-Ser645
Catalog #EGF-CM101
Regular price $225.00
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Product Description
Recombinant Rhesus macaque EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Ser645.
Product Background
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category
Recombinant Protein
Target
EGFR/HER1
Synonyms
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession
Species
Rhesus macaque
Biotinylated
no
Amino Acid Range
Leu25-Ser645
Molecular Weight
The protein has a predicted MW of 69.8 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rhesus macaque EGFR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Rhesus macaque EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque EGFR is greater than 95% as determined by SEC-HPLC.

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