Product Description |
Recombinant Rhesus macaque LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met1-His474. |
Product Background |
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. |
Product Category |
Recombinant Protein |
Target |
LILRB1/CD85j/ILT2 |
Synonyms |
ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4 |
Accession |
|
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Met1-His474 |
Molecular Weight |
The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). The affinity constant of 47.69 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 47.69 nM as determined in SPR assay (Biacore T200). |
|
|
Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). |
|
|
Rhesus macaque LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
