Rhesus macaque LILRB1/CD85j/ILT2 Protein (LIL-CM2B3)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met1-His474

Catalog: LIL-CM2B3

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Product Description Recombinant Rhesus macaque LILRB1/CD85j/ILT2 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Met1-His474.
Product Category Recombinant Protein
Target LILRB1/CD85j/ILT2
Synonyms ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Gene/Protein ID NP_001035762.2
Species Rhesus macaque
Exact Sequence Met1-His474
Molecular Weight The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G Complex Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).
Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 47.69 nM as determined in SPR assay (Biacore T200).
The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC.
Rhesus macaque LILRB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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