Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-CM180B)
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-CM180B)
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-CM180B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp296-Gly580
Catalog #MSL-CM180B
Regular price $577.50
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Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-CM180B) Summary

Product Description

Recombinant Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Asp296-Gly580.

Product Background

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (MSL-CM180B) Specifications/Details

Protein
MSLN/Mesothelin
Synonyms
MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Asp296-Gly580
Molecular Weight
The protein has a predicted MW of 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Biotinylated Cynomolgus MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.
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