Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD27 Ligand (Trimer), His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 67ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu50-Pro193

Catalog: CDL-HM427B

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Product Description Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) is expressed from Expi293 with His tag at the N-terminal.; It contains Leu50-Pro193 Trimer.
Product Category Recombinant Protein
Target CD27 Ligand/CD70 Trimer
Synonyms CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;  Ki-24 antigen; TNFSF7G
Gene/Protein ID P32970-1
Species Human
Exact Sequence Leu50-Pro193
Molecular Weight The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human CD27 Ligand (Trimer), His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 67ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD27 Ligand (Trimer), His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 67ng/ml determined by ELISA.
The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD27 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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