Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)
Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)
Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)
Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer) , His Tag with the EC50 of 9.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu50-Pro193
Catalog #CDL-HM427B
/
Size

Order now, ships by:

Product Description
 Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Leu50-Pro193.
Product Background
 CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Product Category
 Recombinant Protein
Target
 CD27 Ligand/CD70 Trimer
Synonyms
 CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu50-Pro193
Molecular Weight
 The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer) , His Tag with the EC50 of 9.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer) , His Tag with the EC50 of 9.2ng/ml determined by ELISA.
Biotinylated Human CD27 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed