Name: Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)
Brand: KactusBio
SKU: CDL-HM427B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)

Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer), His Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).

Biotinylated Human CD27 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer), His Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu50-Pro193

Catalog #CDL-HM427B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B) Summary

Product Description	Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Leu50-Pro193 trimer design.
Product Background	CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B) Specifications/Details

Protein	CD27 Ligand/CD70
Synonyms	CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G
Accession	P32970-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu50-Pro193
Molecular Weight	The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer), His Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer), His Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).
	Biotinylated Human CD27 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.