Name: Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)
Brand: KactusBio
SKU: CD4-HM440B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)

Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).

Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.

Biotinylated Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.

The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).
Amino Acid Range: Glu21-Arg193

Catalog #CD4-HM440B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B) Summary

Product Description	Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu21-Arg193.
Product Background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B) Specifications/Details

Protein	CD40/TNFRSF5
Synonyms	CD40; CD40L receptor;- TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013;
Accession	P25942
Species	Human
Biotinylated	yes
Amino Acid Range	Glu21-Arg193
Molecular Weight	The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).
	Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.
	Biotinylated Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
	The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.