Name: Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)
Brand: KactusBio
SKU: CD4-HM440B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)

Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).

Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.

Biotinylated Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.

The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test). See testing image for detail.
Amino Acid Range: Glu21-Arg193

Catalog #CD4-HM440B

Size

100UG

500UG

Order now, ships by:

[{"id":42659977199838,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"CD4-HM440B-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human CD40\/TNFRSF5 Protein (CD4-HM440B) - 100UG","public_title":"100UG","options":["100UG"],"price":57750,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584266666206,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"CD4-HM440B-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human CD40\/TNFRSF5 Protein (CD4-HM440B) - 500UG","public_title":"500UG","options":["500UG"],"price":192500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu21-Arg193.
Product Background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	CD40/TNFRSF5
Synonyms	CD40; CD40L receptor;- TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013;
Accession	P25942
Species	Human
Biotinylated	yes
Amino Acid Range	Glu21-Arg193
Molecular Weight	The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).
	Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.
	Biotinylated Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
	The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.