Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test). See testing image for detail.
  • Exact Sequence: Glu21-Arg193

Catalog: CD4-HM440B

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Product Description Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu21-Arg193.

Product Background

 CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.

Product Category

 Recombinant Protein

Target

 CD40/TNFRSF5
Synonyms CD40; CD40L receptor;- TNFRSF5;B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform;CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Gene/Protein ID

 P25942

Species

 Human

Exact Sequence

 Glu21-Arg193
Molecular Weight The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test). See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).
Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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