Biotinylated Human CD47 Protein (CD7-HM447B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.03ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: GIn19-Pro139

Catalog: CD7-HM447B

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Product Description Recombinant Biotinylated Human CD47 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains GIn19-Pro139.
Product Category Recombinant Protein
Target CD47
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Gene/Protein ID Q08722-1
Species Human
Exact Sequence GIn19-Pro139
Molecular Weight The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.03ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.03ug/ml determined by ELISA.
The purity of Biotinylated Human CD47 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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