Product Description |
Recombinant Biotinylated Human CD47 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains GIn19-Pro139. |
Product Background |
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
CD47 |
Synonyms |
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
GIn19-Pro139 |
Molecular Weight |
The protein has a predicted MW of 18.38 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
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Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. |
|
|
Biotinylated Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human CD47 is greater than 95% as determined by SEC-HPLC. |
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