Name: Biotinylated Human CD73/NT5E Protein (CD7-HM473B)
Brand: KactusBio
SKU: CD7-HM473B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve for biotinylated human CD73 His Tag interacting with anti-CD73 antibody, showing dose-dependent OD450 response.

Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.

Biotinylated Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD73 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Trp27-Lys547

Catalog #CD7-HM473B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD73/NT5E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp27-Lys547.
Product Background	CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD73/NT5E
Synonyms	CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Accession	P21589-1
Species	Human
Biotinylated	yes
Amino Acid Range	Trp27-Lys547
Molecular Weight	The protein has a predicted MW of 60.92 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
	Biotinylated Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD73 is greater than 95% as determined by SEC-HPLC.

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