Biotinylated Human CD73/NT5E Protein (CD7-HM473B)
Catalog: CD7-HM473B
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Product Description | Recombinant Biotinylated Human CD73/NT5E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp27-Phe547. |
Product Background |
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases. |
Product Category |
Recombinant Protein |
Target |
CD73/NT5E |
Accession | |
Species |
Human |
Amino Acid Range |
Trp27-Phe547 |
Molecular Weight | The protein has a predicted MW of 60.92 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Liquid |
Shipping | Shipped with dry ice. |
Formulation | Supplied as 0.22 um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). |
Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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