Biotinylated Human CD73/NT5E Protein (CD7-HM473B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Trp27-Lys547
Catalog #CD7-HM473B
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Product Description
 Recombinant Biotinylated Human CD73/NT5E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp27-Lys547.
Product Background
 CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Product Category
 Recombinant Protein
Target
 CD73/NT5E
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Trp27-Lys547
Molecular Weight
 The protein has a predicted MW of 60.92 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD73, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Biotinylated Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD73 is greater than 95% as determined by SEC-HPLC.

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