Product Description |
Recombinant Biotinylated Human CEACAM-5/CD66e Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Lys35-Ala685. |
Product Background |
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated. |
Product Category |
Recombinant Protein |
Target |
CEACAM-5/CD66e |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys35-Ala685 |
Molecular Weight |
The protein has a predicted MW of 74.23 kDa. Due to glycosylation, the protein migrates to 110-150 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. |
|
|
Biotinylated Human CEACAM-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human CEACAM-5 is greater than 95% as determined by SEC-HPLC. |
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