Product Description |
Recombinant Biotinylated Mouse ALCAM/CD166 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr28-Lys527. |
Product Background |
Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
ALCAM/CD166 |
Synonyms |
CD166; ALCAM; MEMD; CD6L; KG-CAM |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Tyr28-Lys527 |
Molecular Weight |
The protein has a predicted MW of 58.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His-Avi Tag with the EC50 of 2.67ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human CD6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His-Avi Tag with the EC50 of 2.67ug/ml determined by ELISA (QC Test). |
|
|
Biotinylated Mouse ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Mouse ALCAM is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from ADC Target Proteins
Recently viewed
