Name: Biotinylated Mouse ALCAM/CD166 Protein (ALC-MM401B)
Brand: KactusBio
SKU: ALC-MM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Mouse ALCAM/CD166 Protein (ALC-MM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His Tag with the EC50 of 1.12ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Tyr28-Lys527

Catalog #ALC-MM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Mouse ALCAM/CD166 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr28-Lys527.
Product Background	Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
Product Category	Recombinant Protein

Target	ALCAM/CD166
Synonyms	CD166; ALCAM; MEMD; CD6L; KG-CAM
Accession	Q61490
Species	Mouse
Biotinylated	yes
Amino Acid Range	Tyr28-Lys527
Molecular Weight	The protein has a predicted MW of 58.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His Tag with the EC50 of 1.12ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His Tag with the EC50 of 1.12ug/ml determined by ELISA.
	Biotinylated Mouse ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse ALCAM is greater than 95% as determined by SEC-HPLC.

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