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Biotinylated Mouse ALCAM/CD166 Protein (ALC-MM401B) Summary
Product Description |
Recombinant Biotinylated Mouse ALCAM/CD166 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr28-Lys527. |
Product Background |
Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) is a glycoprotein involved in homotypic and heterotypic cell adhesion. ALCAM can be proteolytically cleaved at the cell surface by metalloproteases, which generate shedding of its ectodomain. In various tumors, ALCAM is overexpressed and serves as a valuable prognostic marker of disease progression. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Mouse ALCAM/CD166 Protein (ALC-MM401B) Specifications/Details
Protein |
ALCAM/CD166 |
Synonyms |
CD166; ALCAM; MEMD; CD6L; KG-CAM |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Tyr28-Lys527 |
Molecular Weight |
The protein has a predicted MW of 58.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His-Avi Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His-Avi Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). |
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Biotinylated Mouse ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse ALCAM is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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