Cynomolgus FAP Protein (FAP-CM101)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus FAP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu26-Asp760

Catalog: FAP-CM101

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Product Description Recombinant Cynomolgus FAP Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu26-Asp760.

Product Background

Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

Product Category

Recombinant Protein

Target

FAP
Synonyms FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Accession

Species

Cynomolgus

Amino Acid Range

Leu26-Asp760
Molecular Weight The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus FAP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus FAP at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2500-pmol/min/µg (QC Test).
The purity of Cynomolgus FAP is greater than 95% as determined by SEC-HPLC.
Cynomolgus FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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