Human HGF R/c-MET Protein (MET-HM201)
Human HGF R/c-MET Protein (MET-HM201)
Human HGF R/c-MET Protein (MET-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human HGF R, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu25-Thr932
Catalog #MET-HM201
Regular price $195.00
/
Size

Order now, ships by:

Product Description
Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu25-Thr932.
Product Background
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
Product Category
Recombinant Protein
Target
HGF R/c-MET
Synonyms
MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu25-Thr932
Molecular Weight
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human HGF R, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF R, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA.
Immobilized Human HGF, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA (QC Test).
Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed