Human HGF R/c-MET Protein (MET-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human HGF R, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Glu25-Thr932

Catalog: MET-HM201

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Product Description Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Glu25-Thr932.
Product Category Recombinant Protein
Target HGF R/c-MET
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Gene/Protein ID P08581-1
Species Human
Exact Sequence Glu25-Thr932
Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human HGF R, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF R, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test).
Immobilized Human HGF, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 52.8ng/ml determined by ELISA.
Human HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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