Product Description |
Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu25-Thr932. |
Product Background |
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling. |
Product Category |
Recombinant Protein |
Target |
HGF R/c-MET |
Synonyms |
MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu25-Thr932 |
Molecular Weight |
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HGF R, hFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HGF R, hFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA. |
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Immobilized Human HGF, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA (QC Test). |
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Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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