Human IL-3 R alpha/CD123 Protein (IL3-HM1RA)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human IL-3 R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. The affinity constant of 0.276 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr19-Arg305

Catalog: IL3-HM1RA

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Product Description Recombinant Human IL-3 R alpha/CD123 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Thr19-Arg305.
Product Category Recombinant Protein
Target IL-3 R alpha/CD123
Synonyms CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
Gene/Protein ID P26951-1
Species Human
Exact Sequence Thr19-Arg305
Molecular Weight The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-3 R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. The affinity constant of 0.276 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 μM as determined in SPR assay (Biacore T200).
Immobilized Human IL-3 R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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