Product Description |
Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr19-Arg305. |
Product Background |
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
IL-3 R alpha/CD123 |
Synonyms |
CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr19-Arg305 |
Molecular Weight |
The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-3 R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-3 R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). |
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Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC. |
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