Human IL-3 R alpha/CD123 Protein (IL3-HM1RA)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-3 R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr19-Arg305
Catalog #IL3-HM1RA
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Product Description
 Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr19-Arg305.
Product Background
 Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
Product Category
 Recombinant Protein
Target
 IL-3 R alpha/CD123
Synonyms
 CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr19-Arg305
Molecular Weight
 The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-3 R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 uM as determined in SPR assay (Biacore T200).
Immobilized Human IL-3 R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).
Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.

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