Human PTK7/CCK4 Protein (CCK-HM104)

Immobilized Human PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.

Human PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human PTK7 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Amino Acid Range: Ala31-Thr704

Catalog #CCK-HM104

Price

$346⁵⁰

Size

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Human PTK7/CCK4 Protein (CCK-HM104) Summary

Product Description

Recombinant Human PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala31-Thr704.

Product Background

Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation, highlighting the important regulatory role of PTK7 protein. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling.

Human PTK7/CCK4 Protein (CCK-HM104) Specifications/Details

Protein	PTK7/CCK4
Synonyms	CCK-4; PTK7;CCK4
Accession	Q13308-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala31-Thr704
Molecular Weight	The protein has a predicted MW of 75.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
	Human PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PTK7 is greater than 95% as determined by SEC-HPLC.