Mouse LRRC15/LIB Protein (LRR-MM115)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Anti-LRRC15 Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse LRRC15, His Tag with the EC50 of 11.7 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr22-Gly536

Catalog: LRR-MM115

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Product Description Recombinant Mouse LRRC15/LIB Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Tyr22-Gly536.

Product Background

The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Product Category

Recombinant Protein

Target

LRRC15/LIB
Accession

Species

Mouse

Amino Acid Range

Tyr22-Gly536
Molecular Weight The protein has a predicted MW of 58.56 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-LRRC15 Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse LRRC15, His Tag with the EC50 of 11.7 ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-LRRC15 Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse LRRC15, His Tag with the EC50 of 11.7 ng/ml determined by ELISA.
The purity of Mouse LRRC15 is greater than 95% as determined by SEC-HPLC.
Mouse LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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