RNase III (RNI-EE601) Summary
Product Description |
RNase III is a double-stranded RNA (dsRNA) -specific endoribonuclease that can cleave dsRNA into 18-25 bp interfering RNAs (siRNA) with 2 nucleotide 3’ overhangs, 5’ phosphate and 3’ hydroxyl. RNase III can be used to generate siRNA library from long dsRNA for gene silencing and target validation; RNase III can also be used to remove dsRNA, a side product with immunogenicity, that is generated during in vitro transcription. |
Product Category |
Enzyme |
RNase III (RNI-EE601) Specifications/Details
Applications |
Making siRNA library for any target gene Gene silencing and target validation Removal of dsRNA from in vitro transcription |
Concentration |
2U/µL |
Unit Definition |
One unit (U) is defined as the amount of enzyme required to digest 1μg dsRNA into siRNA at 37°C in a 50 μL reaction mixture for 20 minutes. |
Source |
E. coli |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Stability And Storage |
Transport on dry ice. Store at -20 ±5°C for 24 months. Avoid repeated freezing and thawing. |
Validation Images
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Digestion of 1μg 500bp dsRNA with RNase III, followed by Agarose gel analysis. |
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