RNase III (RNI-EE601) Summary
Product Description |
RNase III is a double-stranded RNA (dsRNA) -specific endoribonuclease that can cleave dsRNA into 18-25 bp interfering RNAs (siRNA) with 2 nucleotide 3’ overhangs, 5’ phosphate and 3’ hydroxyl. RNase III can be used to generate siRNA library from long dsRNA for gene silencing and target validation; RNase III can also be used to remove dsRNA, a side product with immunogenicity, that is generated during in vitro transcription. |
Product Category |
Enzyme |
RNase III (RNI-EE601) Specifications/Details
Applications |
Making siRNA library for any target gene Gene silencing and target validation Removal of dsRNA from in vitro transcription |
Concentration |
2U/µL |
Unit Definition |
One unit (U) is defined as the amount of enzyme required to digest 1μg dsRNA into siRNA at 37°C in a 50 μL reaction mixture for 20 minutes. |
Source |
E. coli |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Stability And Storage |
Transport on dry ice. Store at -20 ±5°C for 24 months. Avoid repeated freezing and thawing. |
Validation Images
|
|
Digestion of 1μg 500bp dsRNA with RNase III, followed by Agarose gel analysis. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
