RNase III (RNI-EE601)
Catalog: RNI-EE601
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| Product Description | RNase III is a double-stranded RNA (dsRNA) -specific endoribonuclease that can cleave dsRNA into 18-25 bp interfering RNAs (siRNA) with 2 nucleotide 3’ overhangs, 5’ phosphate and 3’ hydroxyl. RNase III can be used to generate siRNA library from long dsRNA for gene silencing and target validation; RNase III can also be used to remove dsRNA, a side product with immunogenicity, that is generated during in vitro transcription. |
Product Category |
Enzyme |
| Application | Making siRNA library for any target gene Gene silencing and target validation Removal of dsRNA from in vitro transcription |
| Concentration | 2U/µL |
| Unit Definition | One unit (U) is defined as the amount of enzyme required to digest 1μg dsRNA into siRNA at 37°C in a 50 μL reaction mixture for 20 minutes. |
| Source | E. coli |
| Form | Liquid |
| Stability And Storage | Transport on dry ice. Store at -20 ±5°C for 24 months. Avoid repeated freezing and thawing. |
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Digestion of 1μg 500bp dsRNA with RNase III, followed by Agarose gel analysis. |
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RNase III on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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