Name: Biotinylated Human HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer Protein (MHC-HE035B)
Brand: KactusBio
SKU: MHC-HE035B-100UG
Availability: InStock

Biotinylated Human HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer Protein (MHC-HE035B)

Immobilized Biotinylated HLA-A*02:01 MART-1 A27L (ELAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

Biotinylated Human HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated HLA-A*02:01 MART-1 A27L (ELAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide

Catalog #MHC-HE035B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide.
Product Background	Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer
Synonyms	MART1; LB39-AA; SK29-AA; Melan-A; MLANA
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&ELAGIGILTV
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide
Molecular Weight	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated HLA-A*02:01 MART-1 A27L (ELAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated HLA-A*02:01 MART-1 A27L (ELAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
	Biotinylated Human HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 A27L (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.