Product Description |
Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258. |
Product Background |
B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status-. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
B7-H4 |
Synonyms |
B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe29-Ala258 |
Molecular Weight |
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H4, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human B7-H4, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1 ng/ml determined by ELISA. |
|
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Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC. |
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