Human B7-H4 Protein (BH7-HM174)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe29-Ala258

Catalog: BH7-HM174

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Product Description	Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.
Product Background	B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status-.
Product Category	Recombinant Protein

Target	B7-H4
Synonyms	B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291
Gene/Protein ID	Q7Z7D3-1
Species	Human
Exact Sequence	Phe29-Ala258
Molecular Weight	The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
	The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.
	Human B7-H4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.