Human B7-H4 Protein (BH7-HM174)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe29-Ala258

Catalog: BH7-HM174

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Product Description Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.

Product Background

B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status-.

Product Category

Recombinant Protein

Target

B7-H4
Synonyms B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291

Gene/Protein ID

Q7Z7D3-1

Species

Human

Exact Sequence

Phe29-Ala258
Molecular Weight The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.
Human B7-H4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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