Human CXCL13/BCA-1 Protein (CXC-HE113)

  • Express System: E.coli
  • Product Tag: N-His-Sumo
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Anti-CXCL13 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CXCL13, His Tag with the EC50 of 0.54ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val23-Pro109

Catalog: CXC-HE113

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Product Description Recombinant Human CXCL13/BCA-1 Protein is expressed from E.coli with His tag and Sumo tag at the N-Terminus.It contains Val23-Pro109.

Product Background

Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.

Product Category

Recombinant Protein

Target

CXCL13/BCA-1
Accession

Species

Human

Amino Acid Range

Val23-Pro109
Molecular Weight The protein has a predicted MW of 22.9 kDa. The protein migrates to 26-28 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-CXCL13 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CXCL13, His Tag with the EC50 of 0.54ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Sumo

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in 0.1M Glycine, 0.3M NaCl, 0.2M L-arginine, 10% Glycerol (pH 3.0).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-CXCL13 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CXCL13, His Tag with the EC50 of 0.54ug/ml determined by ELISA.
Human CXCL13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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