Human Factor H/CFH Protein (CFH-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Factor H, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser860-Arg1231

Catalog: CFH-HM101

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Product Description	Recombinant Human Factor H/CFH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser860-Arg1231.
Product Background	Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack factor H to evade the immune response.
Product Category	Recombinant Protein

Target	Factor H/CFH
Synonyms	Complement factor H; H factor 1; CFH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HUS
Accession	P08603-1
Species	Human
Amino Acid Range	Ser860-Arg1231
Molecular Weight	The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Factor H, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Factor H, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
	The purity of Human Factor H is greater than 95% as determined by SEC-HPLC.
	Human Factor H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.