Product Description |
Recombinant Human Factor H/CFH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser860-Arg1231. |
Product Background |
Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack factor H to evade the immune response. |
Product Category |
Recombinant Protein |
Target |
Factor H/CFH |
Synonyms |
Complement factor H; H factor 1; CFH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HUS |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser860-Arg1231 |
Molecular Weight |
The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Factor H, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Factor H, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. |
|
|
Human Factor H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human Factor H is greater than 95% as determined by SEC-HPLC. |
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