Human Factor H/CFH Protein (CFH-HM101)
Human Factor H/CFH Protein (CFH-HM101)
Human Factor H/CFH Protein (CFH-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Factor H, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser860-Arg1231
Catalog #CFH-HM101
Regular price $297.00
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Human Factor H/CFH Protein (CFH-HM101) Summary

Product Description

Recombinant Human Factor H/CFH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser860-Arg1231.

Product Background

Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack factor H to evade the immune response.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human Factor H/CFH Protein (CFH-HM101) Specifications/Details

Protein
Factor H/CFH
Synonyms
Complement factor H; H factor 1; CFH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HUS
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser860-Arg1231
Molecular Weight
The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Factor H, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Factor H, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). Immobilized Human Factor H, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test).
Human Factor H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Factor H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Factor H is greater than 95% as determined by SEC-HPLC. The purity of Human Factor H is greater than 95% as determined by SEC-HPLC.
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