Human Factor H/CFH Protein (CFH-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Factor H, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser860-Arg1231

Catalog: CFH-HM101

$270.00
Size:

Order now get it on

Product Description Recombinant Human Factor H/CFH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser860-Arg1231.

Product Background

Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack factor H to evade the immune response.

Product Category

Recombinant Protein

Target

Factor H/CFH
Synonyms Complement factor H; H factor 1; CFH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HUS

Gene/Protein ID

P08603-1

Species

Human

Exact Sequence

Ser860-Arg1231
Molecular Weight The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Factor H, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Factor H, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.
The purity of Human Factor H is greater than 95% as determined by SEC-HPLC.
Human Factor H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in