Product Description |
Recombinant Human PODXL2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly33-Thr500. |
Product Background |
Transmembrane protein endoglycan (PODXL2), which belongs to the CD34-family of highly glycosylated sialomucins. Endoglycan is broadly expressed in the developing mouse brains and is proteolytically shed in vitro in mouse neurons and in vivo in mouse brains. Endoglycan shedding in primary neurons was mediated by the transmembrane protease a disintegrin and metalloprotease 10 (ADAM10), but not by its homolog ADAM17. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
PODXL2 |
Synonyms |
Endoglycan; PODLX2; brain-1; BRN1; EG; oct-8; OTF8; UNQ1861/PRO3742 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly33-Thr500 |
Molecular Weight |
The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 85-115 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human PODXL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human PODXL2 is greater than 95% as determined by SEC-HPLC. |
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