Mouse APOE/Apolipoprotein E Protein (APO-MM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu19-Gln311
Catalog #APO-MM102
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Product Description
 Recombinant Mouse APOE/Apolipoprotein E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu19-Gln311.
Product Background
 Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
Product Category
 Recombinant Protein
Target
 APOE/Apolipoprotein E
Synonyms
 Apolipoprotein E; Apo-E; APOE; apolipo E
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Glu19-Gln311
Molecular Weight
 The protein has a predicted MW of 35.1 kDa same as Bis-Tris PAGE result.
Activity
 The affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).
Mouse APOE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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