RNase H (RNH-EE101)
Catalog: RNH-EE101
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| Product Description | RNase H (Ribonuclease H) is an endoribonuclease that can specifically hydrolyze the phosphodiester bonds of RNA and degrades the RNA strand in the RNA-DNA hybrid. RNase H cannot hydrolyze phosphodiester bonds in single- or double-stranded DNA. |
| Application | Remove RNA from RNA-DNA hybrid Oligodeoxyribonucleotide-directed cleavage of RNA Remove poly(A) from mRNA in the presence of oligo(dT) |
| Concentration | 5U/µL |
| Unit Definition | One unit (U) is defined as the amount of enzyme required to generate 1 nmol of ribonucleotides from 20 pmol of RNA-DNA hybrid in a 50 µL reaction within 20 minutes at 37°C. |
| Source | E. coli |
| Form | Liquid |
| Stability And Storage | Transport on dry ice. Store at -20 ±5°C for 24 months. Avoid repeated freezing and thawing. |
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Digestion of an RNA-DNA hybrid substrate with RNase H, followed by Agarose gel analysis. |
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RNase H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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