• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Clusterin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Asp23-Glu449
Catalog #CLN-HM101
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Human Clusterin Protein (CLN-HM101) Summary

Product Description

 Recombinant Human Clusterin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu449.

Product Background

 Clusterin (CLU) is a stress-activated, ATP-independent molecular chaperone, normally secreted from cells, that is up-regulated in Alzheimer disease and in many cancers. It plays important roles in protein homeostasis/proteostasis, inhibition of cell death pathways, and modulation of pro-survival signalling and transcriptional networks. Changes in the CLU gene locus are highly associated with Alzheimer disease, and many therapy-resistant cancers over-express CLU.

Human Clusterin Protein (CLN-HM101) Specifications/Details

Protein
 Clusterin
Synonyms
 Clusterin; Apo-J; Clustrin; SGP-2; Msgp-2; CLU; AAG4; APOJ; CLI; KUB1; SP-40; TRPM2; CLI; KUB1;;SGP2; Msgp2; SP40
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp23-Glu449
Molecular Weight
 The protein has a predicted MW of 51.2 kDa. The protein migrates to 38-41 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Clusterin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Clusterin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). Immobilized Human Clusterin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
Human Clusterin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Clusterin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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