• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.330 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Leu19-Thr138
Catalog #NKP-HM430B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430B) Summary

Product Description

 Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138.

Product Background

 NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells.

Biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430B) Specifications/Details

Protein
 NKp30/NCR3/CD337
Synonyms
 CD337; LY117; NCR3; NKp30; 1C7; MALS
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu19-Thr138
Molecular Weight
 The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.330 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 uM as determined in SPR assay (Biacore T200). Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
Biotinylated Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...